Annole is a tall and slender reptilian creature with a pale blue-green skin tone. She has long, straight cyan hair with bangs. She has large hands and feet with three fingers/toes each, long slender arms and legs, a rounded nose, and a long tail.
She appears to be wearing either glasses or thick eyeliner. She has sharp teeth with one sharp tooth usually poking out of her mouth. She wears a dark gray short sleeved crop top, red capri pants, and a backwards red newsboy hat.
In "Plaza Prom", she wears a red dress, her backwards red newsboy cap, and either no glasses or thinner eyeliner.
- "Seasons Change" (flashback)
- Annole, alongside the rest of the Lizard Gang (excluding Chameleon Jr.), was designed by revisionist and storyboard artist, Anna Craig.
- In "We've Got Fleas", Annole is seen with the Lizard Gang being members of the unnamed Human-based subculture.
- Her name is a cross between the species of lizard she is based on, the anole, and the fairly common English female name, "Anne" or "Anna", quite possibly after her designer, Anna Craig.